Samuel Fröler originally worked for a clinic specialized in adolescent psychiatry before he decided to become an actor. He attended the drama school in Göteborg and was a member of the ensemble of Stockholm's City Theatre for several years. Fröler's breakthrough came with the TV series "Tre Kärkelar", where he played an officer of the Swedish air force. Impressed by his performance, Ingmar Bergman engaged Fröler to take the role of the director's father in Bergman's autobiographical film "The Best Intentions"(1992). "The Best Intentions" received the Palme d'Or in Cannes and Fröler was nominated for the prestigious Swedish film prize “Gold Bug” in the category “Best Actor”.

Fröler has starred in a great number of Scandinavian and international films and is noted for his appearances in "Pure" and in the TV series "Seks Som Oss". Moreover, he is known as the Swedish dubbing actor of Shrek in the homonymous animation series and has released his own music album, which had lots of success and made it to the Swedish charts.